Une pure merveille !
Un roman d'une grande beauté, drôle, fin, extrêmement lumineux sur des sujets difficiles : la perte de
l'être aimé, la dureté de la vie et la tristesse qu'on barricade parfois... Elise franco-japonaise,
orpheline de sa maman veut poser LA question à son père et elle en trouvera le courage au fil des pages,
grâce au retour de sa grand-mère du japon, de sa rencontre avec son extravagante amie Stella..
Ensemble il ne diront plus Sayonara mais Mata Ne !
This laboratory manual gives a thorough introduction to modern, cost efficient techniques in Molecular Biology, which can find application in many different...
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Résumé
This laboratory manual gives a thorough introduction to modern, cost efficient techniques in Molecular Biology, which can find application in many different fields. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses and workshops or tested in the author's laboratory. Thus, they are also likely to work in inexperienced hands the first time they are performed. Step-by-step protocols and practical notes are provided. The distinguishing feature of this manual compared to others is the detailed explanation of the theoretical mechanisms of each step and the discussion of the importance of the techniques. In addition, each protocol includes an indication of the time and expense involved in its application. This knowledge will enable the users to design their own modifications or to adapt the method to different systems. Dr Surzycki has been teaching undergraduate courses and leading workshops in introductory Molecular Biology for many years, during which time he has extensively modified and refined the techniques he has described in this manual.
Sommaire
General aspect of DNA isolation and purification
Preparation of genomic DNA from animal cells
Preparation of genomic DNA from plant cells
Preparation of genomic DNA from bacteria
Isolation of plasmid DNA
Isolation and purification of RNA
Isolation of polyA + RNA
Agarose gel electrophoresis of DNA
Preparation of probes for hybridization
Nucleic acid hybridization : a theoretical consideration