Protein Purification Applications. 2nd Edition

Major advances have occurred in protein purification techniques over the past ten years with very substantial improvements in equipment control, automation, and separation materials, as well as the introduction of new techniques such as affinity membranes and expanded beds. The central importance of protein purification has merited two new companion books in the Practical Approach Series. Each book contains detailed practical advice and step-by-step methods aimed at laboratory scale purification, with additional information on scale-up where appropriate. Together the books cover the key approaches and techniques, plus their successful application in common situations, with helpful advice on how to extend or adapt the methodology to user specific situations. Protein Purification Applications describes strategies and detailed practical procedures for protein purification from common sources: mammalian cell culture, microbial cell culture, animal tissue, plant tissue, and milk. It also includes chapters on the purification of inclusion bodies, fusion proteins, and of proteins for crystallography. The ultimate test of a purification strategy is to yield a single highly pure protein from .in initial crude mixture of proteins, carbohydrates, lipids, and cells debris. These two volumes are designed to give researchers all the information, protocols and practical advice needed to achieve a successful outcome. Protein Purification Techniques focuses on unit operations and analytical techniques. It starts with an overview of purification strategy and then covers initial extraction and clarification techniques. The rest of the book concentrates on a range of key purification methods with the emphasis being on chromatography. The final chapter considers scale-up considerations.