With the "post genomics" era comes an increasing demand for the techniques of cell biology which are critical to the interpretation of the location and function of the myriad of proteins within a cell. In response, this second edition of Plant Cell Biology balances established techniques with major new developments in the field. The book covers a wide range of methods, including classical histochemistry, electron microscopy, cytometry, micromanipulation and electrophysiological techniques, the isolation of cytoplasmic organelles, application of fluorescent probes, expression systems, the use of green fluorescent protein, and macromolecular location procedures involving immunocytochemistry and in situ hybridization. In summary, this volume is a comprehensive laboratory manual of key approaches and techniques, supplemented with a great deal of wise advice for success. It provides advanced students, postgraduates and researchers in the plant sciences with an invaluable companion and guide to the ever-growing field of plant cell biology.