Plant Cell Biology. A Practical Approach, 2nd Edition

Béatrice Satiat-Jeunemaitre

,

Chris Hawes

Note moyenne 
Béatrice Satiat-Jeunemaitre et Chris Hawes - Plant Cell Biology. A Practical Approach, 2nd Edition.
With the "post genomics" era comes an increasing demand for the techniques of cell biology which are critical to the interpretation of the location and... Lire la suite
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Résumé

With the "post genomics" era comes an increasing demand for the techniques of cell biology which are critical to the interpretation of the location and function of the myriad of proteins within a cell. In response, this second edition of Plant Cell Biology balances established techniques with major new developments in the field. The book covers a wide range of methods, including classical histochemistry, electron microscopy, cytometry, micromanipulation and electrophysiological techniques, the isolation of cytoplasmic organelles, application of fluorescent probes, expression systems, the use of green fluorescent protein, and macromolecular location procedures involving immunocytochemistry and in situ hybridization. In summary, this volume is a comprehensive laboratory manual of key approaches and techniques, supplemented with a great deal of wise advice for success. It provides advanced students, postgraduates and researchers in the plant sciences with an invaluable companion and guide to the ever-growing field of plant cell biology.

Sommaire

    • Introduction to optical microscopy for plant cell biology
    • Fluorescent probes for living plant cells
    • Flow cytometry
    • Transient expression, a tool to address questions in plant cell biology
    • The green fluorescent protein (GFP) as reporter in plant cells
    • Microinjection
    • Micromanipulation by laser microbeam and optical tweezers
    • Electrophysiological methods: monitoring exo- and endocytosis in real time
    • Plant histology
    • Immunocytochemistry for light microscopy
    • Electron microscopy
    • In situ hybridization
    • Organelle isolation

Caractéristiques

  • Date de parution
    07/06/2001
  • Editeur
  • ISBN
    0-19-963865-9
  • EAN
    9780199638659
  • Présentation
    Broché
  • Nb. de pages
    338 pages
  • Poids
    0.805 Kg
  • Dimensions
    18,9 cm × 24,6 cm × 2,0 cm

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