Gradient HPLC for Practitioners - RP, LC-MS, Ion Analytics, Biochromatography, SFC, HILIC - Grand Format

Edition en anglais

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This practical guide for analytical scientists explains the use of gradients in liquid chromatography, which account for the majority of chromatographic... Lire la suite
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Résumé

This practical guide for analytical scientists explains the use of gradients in liquid chromatography, which account for the majority of chromatographic separations. After a description of the fundamentals of gradient separations, the most common application scenarios are addressed, from LC-MS coupling to biochromatography to the separation of ionic substances. Throughout, the book provides detailed, practical information for practitioners, enabling them to use gradient separation methods reliably and efficiently.
Gradient HPLC for Practitioners : RP, LC-MS, Ion Analytics, Biochromatography, SFC, HILIC starts by examining aspects of gradient optimization - looking at special features of the gradient, some chromatographic definitions and formulas, and a set of easy-to-remember rules of thumb when setting up a gradient HPLC. It then provides readers with both linear and curvilinear models to study the prediction of gradients.
Next, it examines the requirements, optimal use, hints, and pitfalls of the gradient itself- presenting instrumental influences in gradient elution, gradient elution technology, and ways to systematically characterize gradient instrumentation. Other topics covered include use of gradients in LC-MS ; specifications of gradients in biochromatography ; the gradient in the separation of ionic components ; use of gradients in hydrophilic interaction liquid chromatography (HILIC) ; gradient elution in supercritical fluid chromatography (SFC) and the specifics of flow and temperature gradients.
Captures the experience and technological advances in gradient HPLC of the past decade. Addresses everyday analytical challenges of novice and routine users. Examines the detection limit, peak capacity, and resolution in gradient optimizations, as well as gradient "myths" and examples for optimizing gradient runs. Gradient HPLC for Practitioners : RP, LC-MS, Ion Analytics, Biochromatography, SFC, HILIC will appeal to analytical chemists, chromatographers, environmental chemists, food chemists, forensic scientists, and pharmaceutical chemists.

Caractéristiques

  • Date de parution
    17/04/2019
  • Editeur
  • ISBN
    978-3-527-34408-6
  • EAN
    9783527344086
  • Format
    Grand Format
  • Présentation
    Relié
  • Nb. de pages
    230 pages
  • Poids
    0.631 Kg
  • Dimensions
    17,7 cm × 24,9 cm × 2,7 cm

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À propos de l'auteur

Biographie de Stavros Kromidas

Stavros Kromidas studied biology and chemistry at the University of Saarbrücken, where in 1983 he obtained his PH. D. degree on the development of new chiral stationary phases for HPLC. After working for Waters GmbH for five years, he founded NO VIA GmbH, a provider of professional training and consulting in analytical chemistry, serving as the CEO until 2001. Since 2001 he works a an independent consultant for analytical chemisay, based in Blieskastel (Germany).
For more than 20 years he has regularly held lectures and training courses on OPEC, and has authored numerous articles and several books on various aspects of chromatography.

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