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Electrophoresis In Practice. A Guide To Methods And Applications Of Dna And Protein Separations, Third Edition
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Résumé
This is your "Laboratory Guide" for successful electrophoretic separations ! Organized in two parts it gives the reader a thorough presentation of the fundamentals followed by a detailed description of 15 of the most common methods currently in use. The Third Edition now includes the latest developments in 2D-electrophoresis as well as an overview of the proteome analysis methodology.
Sommaire
- FUNDAMENTALS
- Electrophoresis
- Isotachophoresis
- Isoelectric focusing
- Blotting
- Interpretation of electropherograms
- Proteome Analysis
- Instrumentation
- METHODS
- PAGE of dyes
- Agarose and immuno electrophoresis
- Titration curve analysis
- Native PAGE in amphoteric buffers
- Agarose IEF
- PAGIEF in rehydrated gels
- Horizontal SDS-PAGE
- Vertical PAGE
- Semi-dry blotting of proteins
- IEF in immobilized pH gradients
- High-resolution 2D electrophoresis
- PAGE of double stranded DNA
- Native PAGE of single stranded DNA
- Denaturing gradient gel electrophoresis
- Denaturing PAGE of DNA
Caractéristiques
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Date de parution06/04/2001
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Editeur
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ISBN3-527-30300-6
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EAN9783527303007
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PrésentationRelié
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Nb. de pages349 pages
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Poids0.85 Kg
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Dimensions17,7 cm × 24,5 cm × 2,5 cm
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