Animal cell culture is central to much of the current research in the life sciences, as shown by the major success of previous volumes in the Practical Approach Series on this topic. This new edition has been fully updated. As well as covering fundamental material for those new to the subject, including basic principles, serum-free culture, characterisation and authentication, it has improved and new chapters on three-dimensional culture, stem cell assays, scale-up for commercial production of proteins, FISH, genetic modification, tissue engineering, apoptosis assays and cytotoxicity assays. Detailed protocols provide the core of each chapter and are presented in an easy to follow, step by step format, making the new methodology readily accessible. All researchers who work in biological or medical areas and need to use cell culture, whether in academia or in a commercial organisation, will benefit greatly from this book.